生长激素受体基因突变对西藏藏猪生长迟缓的影响

doi:10.11843/j.issn.0366-6964.2016.05.004

Abstract

Abstract:

This study aimed to explore the genetic and molecular mechanism of Tibet mini-pig growth retardation.RT-PCR technique was used to amplify Tibet mini-pig GHR gene.Bioinformatics method was used to analyze the differences of GHR gene and protein sequence between Tibet mini-pig and other common variety pigs.The eukaryotic expression vector of common pig GHR gene was transfected into Tibet mini-pig’s embryonic fibroblasts.The MTT assay was used to detect the cell proliferation activity after transfected GHR-pIRES2-EGFP vector.The real-time PCR was used to detect the mRNA expression of the IGF-1 gene.There were 1 716 bp in Tibet mini-pig GHR gene coding region，and 572 amino acids were encoded.Compared with common meat pig GHR gene sequence，the base T in Tibet mini-pig GHR gene coding region 1 225 bp mutated into G，and then serine was replaced by alanine.The growth activity of Tibet mini-pig’s embryonic fibroblasts were significantly improved after transfected with GHR-pIRES2-EGFP，and the mRNA level of IGF-1 was up-regulated significantly.The results showed that GHR could improve the activity of PEFs cell proliferation，and induce the expression of IGF-1 gene；The mutation of GHR gene in 1 225 bp might involve in the process of growth retardation in Tibet mini-pig.

CLC Number:

S828.2

YUE Min，TIAN Yu-guang，WAN Bin，PANG Wei，WU Qing-hong，WANG Yu-jue. The Impact of GHR Mutation on Suppressing the Growth in Tibet Mini-pig[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 882-887.

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The Impact of GHR Mutation on Suppressing the Growth in Tibet Mini-pig

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